Table II.
Mean Residue Ellipticity (MRE) Values at pH 5.8 for TM4-CxCT Domains
| Isoform | TFE (%) | First local minimum
|
Minimum at 222 nm
|
Totala/CT-only
|
|
|---|---|---|---|---|---|
| Wavelength (nm) | MRE | MRE | α-helical content (%/%) | ||
| TM4-Cx26CT | 0 | 208.8 | −37,208.4 | −33,569.4 | 64.9/18.6 (1.7)b (2.3)* |
| 5 | 208.7 | −39,574.6 | −35,057.7 | 67.1/20.8 | |
| 10 | 209.8 | −40,799.1 | −38,041.4 | 66.3/20.0 | |
| 15 | 208.7 | −43,163.2 | −37,000.5 | 68.9/22.6 | |
| 30 | 209.2 | −45,769.4 | −42,092.3 | 70.8/24.5 (2.2) | |
| TM4-Cx32CT | 0 | 208.1 | −14,522.3 | −11,797.9 | 36.9/11.7 (7.8) |
| 5 | 208.7 | −14,788.3 | −12,027.7 | 35.8/10.6 | |
| 10 | 208.5 | −14,269.4 | −11,587.9 | 35.3/10.1 | |
| 15 | 208.3 | −15,702.2 | −13,142.4 | 36.9/11.7 | |
| 30 | 208.7 | −15,476.2 | −13,553.2 | 37.8/12.6 (8.4) | |
| TM4-Cx37CT | 0 | 208.0 | −10,564.9 | −7909.7 | 22.6/3.0 (3.0) |
| 5 | 208.0 | −11,181.2 | −8517.3 | 22.0/2.4 | |
| 10 | 208.1 | −11,175.5 | −8465.8 | 22.8/3.2 | |
| 15 | 208.1 | −11,523.4 | −8827.0 | 23.3/3.7 | |
| 30 | 208.1 | −13,356.5 | −10,210.9 | 21.0/1.4 (1.4) | |
| TM4-Cx40CT | 0 | 208.5 | −4513.9 | −3639.9 | 14.8/0.0 (0.0) |
| 5 | 208.1 | −4650.1 | −3685.8 | 14.4/0.0 | |
| 10 | 208.7 | −5670.0 | −4598.4 | 16.5/0.0 | |
| 15 | 208.7 | −5386.1 | −4439.3 | 16.1/0.0 | |
| 30 | 207.8 | −5625.9 | −4617.1 | 18.2/1.9 (2.4) | |
| TM4-Cx43CT | 0 | 207.4 | −9620.9 | −6806.9 | 21.5/7.0 (10.4) |
| 5 | 207.6 | −11,481.8 | −8170.9 | 24.9/10.4 | |
| 10 | 207.7 | −11,117.2 | −7972.6 | 26.4/11.9 | |
| 15 | 207.6 | −11,416.0 | −8297.6 | 26.3/11.8 | |
| 30 | 207.6 | −12,999.3 | −9998.5 | 27.3/12.8 (19.0) | |
| TM4-Cx45CT | 0 | 208.3 | −11,067.3 | −9079.7 | 28.4/12.9 (18.1) |
| 5 | 208.0 | −10,850.7 | −8788.0 | 27.4/11.9 | |
| 10 | 208.3 | −11,478.2 | −9366.4 | 29.1/13.6 | |
| 15 | 208.1 | −12,276.9 | −9981.2 | 29.9/14.4 | |
| 30 | 208.4 | −14,936.6 | −12,477.7 | 36.9/21.4 (30.0) | |
| TM4-Cx50CT | 0 | 207.4 | −4316.78 | −3204.4 | 12.4/0.9 (1.8) |
| 5 | 206.9 | −4655.57 | −3426.1 | 14.1/2.6 | |
| 10 | 206.4 | −4773.65 | −3514.0 | 13.5/2.0 | |
| 15 | 207.2 | −4909.53 | −3732.5 | 13.9/2.4 | |
| 30 | 208.8 | −5771.22 | −4716.1 | 17.1/5.6 (11.4) | |
a
Determined using DichroWeb.
b
Estimate of the total number of CT residues structured as an α-helix. The contribution of the TM4 α-helix was subtracted from the total α-helix percentage, leaving the remaining α-helical content from the CT domain.