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MAF protein was heterogeneously expressed and only partially correlated with mRNA levels in MM cells. Cell lysates were isolated from HMCLs harboring t(14;16), t(4;14), or cell lines without these translocations and resolved on 8% SDS-polyacrylamide gels. Protein was transferred to membranes and blotted with anti-MAF antibody using immunoblotting analysis (A). The level of MAF mRNA in HMCLs was determined by RT-qPCR (B).
