Figure 7. Proteasome inhibitor MG-132 and suppression of treacle in A549 cell response to H₂O₂.

A549 cells exposed 1 mM H₂O₂ for 6 hr (lane 3) had reduced treacle compared to controls (lane 1). However, treacle did not decline if cells were first incubated with 10 MG-132 μM for 10 min prior to H₂O₂ exposure (lane 4) although the level was less than in cells treated with the inhibitor and not exposed to H₂O₂ (lane 2). These data are representative of three independent experiments.