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Predicted consequences of the changes in FLOT1 mRNA sequences at the protein level: frame shift and early stop of translation. ExPasy Translate tool was used to convert the sequencing data to protein sequences. (a) WT flotillin-1 protein sequence with the same exon color coding as in Figure 5. The codons for the residues marked in red are compiled from the flanking exons; (b) clone 2: removal of exons 3–5 results in a frame shift and early stop; as does (c) the removal of exon 3.
