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. 2014 Aug 11;3(8):e113. doi: 10.1038/oncsis.2014.26

Figure 4.

Figure 4

Relationship between miR-206 and cyclin D1 expression in normal and cancer prostate cells. RNA was prepared from LNCaP, DU145, PC-3 and PrEC cells. (a) Levels of miR-206 were determined by real-time quantitative PCR (RT-qPCR). RNU24 levels were used as a loading control. Values represent mean±s.e.m. (b) Protein extracts were prepared from LNCaP, PC-3, DU145 and PrEC cells and analyzed by western blot for cyclin D1 expression. Beta-actin served as a loading control.