Skip to main content
. 2016 Jun 17;7(28):44630–44643. doi: 10.18632/oncotarget.10134

Figure 3. Piezo2 knockdown affects angiogenesis and vascular permeability in vivo.

Figure 3

A. The retinas of C57BL/6 mice were injected with scrambled shRNA (Negative control, NC) or Piezo2 shRNA at P1 and P3 (n=5 animals per group). Isolectin B4 staining and quantification of vascuarization was conducted at P3 and P5. Scale bar, 200 μm. B. Isolectin B4 stained tip cells and filopodia at P5 and quantification of tip cells and filopodia numbers was conducted (n=5 animals per group). Scale bar, 50 μm. C. Hydron pellets containing VEGF (50 ng) or IL-1β (10 ng) were implanted into the corneas of C57BL/6 mice (Piezo2 knockdown group and NC group). On day 5, corneal neovasculature was monitored by slit lamp and neovasculature area was quantified (n=6 animals per group, *P<0.05, Piezo2 knockdown group versus NC group). (C) VEGF (30 ng) or IL-1β (10 ng) was administered to C57BL/6 mice intradermally on the ear, and then injected with Piezo2 shRNA and scrambled shRNA (NC). Evans blue dye was injected and circulated for 30 min. Evans blue was extracted and the concentration was determined. A representative image was shown (n=6 animals per group, *P<0.05, Piezo2 knockdown group versus NC group). All data were from three independent experiments.