Abstract
We studied the adsorption of human albumin onto synthetic hydroxyapatite, using a radiotracer technique and a special flow cell. Adsorption was studied under various conditions corresponding to different thermodynamic paths. It appears that (i) as is the usual case, the isotherms obtained within a short time range (a few hours) do not correspond to a true equilibrium situation; (ii) when the adsorption process is followed for longer times, which is necessary at low bulk concentrations, one always reaches the plateau surface adsorption; (iii) this plateau value is independent of the "history" of the adsorption process and corresponds well to the jamming limit predicted by the random sequential adsorption model; and (iv) surface denaturation, leading to enhanced surface binding and thus decreasing desorption constants, is the important phenomenon that can partly and qualitatively explain our observations. Its time dependence, however, remains to be clarified.
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- Gorbunoff M. J. The interaction of proteins with hydroxyapatite. I. Role of protein charge and structure. Anal Biochem. 1984 Feb;136(2):425–432. doi: 10.1016/0003-2697(84)90239-2. [DOI] [PubMed] [Google Scholar]
- Gorbunoff M. J. The interaction of proteins with hydroxyapatite. II. Role of acidic and basic groups. Anal Biochem. 1984 Feb;136(2):433–439. doi: 10.1016/0003-2697(84)90240-9. [DOI] [PubMed] [Google Scholar]
- Gorbunoff M. J., Timasheff S. N. The interaction of proteins with hydroxyapatite. III. Mechanism. Anal Biochem. 1984 Feb;136(2):440–445. doi: 10.1016/0003-2697(84)90241-0. [DOI] [PubMed] [Google Scholar]
- HJERTEN S., LEVIN O., TISELIUS A. Protein chromatography on calcium phosphate columns. Arch Biochem Biophys. 1956 Nov;65(1):132–155. doi: 10.1016/0003-9861(56)90183-7. [DOI] [PubMed] [Google Scholar]
- McFarlane A. S. IN VIVO BEHAVIOR OF I-FIBRINOGEN. J Clin Invest. 1963 Mar;42(3):346–361. doi: 10.1172/JCI104721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizutani T., Mizutani A. Prevention of adsorption of protein on controlled-pore glass with amino acid buffer. J Chromatogr. 1975 Aug 20;111(1):214–216. doi: 10.1016/s0021-9673(01)80166-7. [DOI] [PubMed] [Google Scholar]
- Talbot J, Tarjus G, Schaaf P. Unexpected asymptotic behavior in random sequential adsorption of nonspherical particles. Phys Rev A Gen Phys. 1989 Oct 15;40(8):4808–4811. doi: 10.1103/physreva.40.4808. [DOI] [PubMed] [Google Scholar]