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. 2016 Dec 28;27(1):87–98. doi: 10.1093/glycob/cww078

Fig. 3.

Fig. 3.

The cryptococcal UDP-Gal transporter localizes with a Golgi marker and is functional in mammalian cells. (A) MDCKII cells transiently transfected with vector alone (vector) or vector expressing HA-tagged forms of the human UDP-Gal transporter (hUgt) or cryptococcal UDP-Gal transporter (cUgt1) were probed with antibodies to HA and to the Golgi protein giantin (as indicated above). As expected, all cells stain with the Golgi marker, while a subset expresses each colocalizing transporter. (B) Lec8 cells were transiently transfected with the same constructs as in Panel A and probed with anti-HA antibody and GSII-FITC (a lectin specific for terminal GlcNac) (denoted above). Terminal galactose modification of cell surface glycans, which prevents the lectin binding, occurs only in cells that express a UDP-Gal transporter. Bright field and merged images are shown (Panel A scale bar, 10 μm; Panel B scale bar, 20 μm). α-HA, red; α-Giantin and GSII, green. This figure is available in black and white in print and in color at Glycobiology online.