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. 1991 Jul 1;88(13):5809–5813. doi: 10.1073/pnas.88.13.5809

Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein.

B M Curtis 1, D E Williams 1, H E Broxmeyer 1, J Dunn 1, T Farrah 1, E Jeffery 1, W Clevenger 1, P deRoos 1, U Martin 1, D Friend 1, et al.
PMCID: PMC51967  PMID: 1829529

Abstract

Granulocyte/macrophage colony-stimulating factor-interleukin 3 (GM-CSF-IL-3) fusion proteins were generated by construction of a plasmid in which the coding regions of human GM-CSF and IL-3 cDNAs were connected by a synthetic linker sequence followed by subsequent expression in yeast. Both GM-CSF-IL-3 and IL-3-GM-CSF fusion proteins were purified to homogeneity and shown to bind to cell-surface receptors through either their GM-CSF or IL-3 domains. The fusion proteins exhibited enhanced receptor affinity, proliferative activity, and hematopoietic colony-stimulating activity compared with either IL-3 and/or GM-CSF alone. This suggests that GM-CSF-IL-3 fusion proteins may hold future promise as therapeutic agents.

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Selected References

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