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. 1991 Jul 1;88(13):5912–5916. doi: 10.1073/pnas.88.13.5912

Analysis of Krüppel control elements reveals that localized expression results from the interaction of multiple subelements.

Y Jacob 1, S Sather 1, J R Martin 1, R Ollo 1
PMCID: PMC51988  PMID: 1905819

Abstract

The Drosophila gap gene Krüppel (Kr) displays a complex spatiotemporal pattern of expression during embryogenesis. Using P-element transformation experiments, we demonstrate that control elements guiding Kr expression in the central or in the anterior domain at the blastoderm stage are each composed of multiple subelements that interact synergistically. We provide evidence that bicoid (bcd) and hunch-back (hb) gene products, as well as at least one other activator, are needed to activate Kr expression in the central domain. We localize and describe regulatory elements within the 4.1-kilobase region proximal to the Kr promoter that are responsible for expression in the ectoderm, mesoderm, amnioserosa, and nervous system. Finally, a protein instability motif encoded in the second exon appears to be important for resetting the dynamic Kr pattern.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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