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. 1994 Feb 1;91(3):893–896. doi: 10.1073/pnas.91.3.893

A simpler sort of antibody.

L Masat 1, M Wabl 1, J P Johnson 1
PMCID: PMC521418  PMID: 8302862

Abstract

The monoclonal antibody NEMO is directed against a molecule expressed by human cells of the melanocytic lineage. Although obtained by conventional immunization and fusion procedures, NEMO consists solely of kappa light chain. SDS/PAGE analysis indicates that the kappa chains are present as both monomers and dimers. When these two forms were separated by gel filtration, only the monomeric form bound antigen. As kappa light chains from the myeloma MOPC-41 and the hybridoma MORK do not bind to the melanocytic cells, we conclude that the binding specificity of NEMO resides in the variable region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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