Figure 7. Rab7 participated in FOXO1-mediated autophagy in Aldo-induced podocytes.

A. Real-time RT-PCR analysis revealed the expression of Rab5 and Rab7 mRNAs normalized with GAPDH after various treatments, as indicated. B. Equal numbers of podocytes were incubated in media containing buffer (control), siRNA-Con (20 nM) or siRNA-FOXO1 (20 nM) with or without 10-7 M Aldo for 24 h, as indicated. The whole cell lysate was immunoblotted with antibodies against Rab5, Rab7, and β-actin. C. Graphical presentation indicates the relative abundance levels of Rab5 and Rab7 after normalization with β-actin. D. Equal numbers of podocytes were incubated in media containing buffer (control), siRNA-Con (20 nM) or siRNA-Rab7 (20 nM) with or without 10-7 M Aldo for 24 h, as indicated. E. Graphical presentation indicates the relative abundance levels of LC3II/LC3I, p62, Rab7, CHOP, Nephrin, and Podocin after normalization with β-actin. F. After the various indicated treatments, podocytes were stained with Annexin V and PI and then analyzed by flow cytometry. Quantification of apoptotic cells by flow cytometry. Results (means±SEM) of 3 series of experiments. #P<0.05 vs. normal control, *P<0.05 vs. Aldo alone or Aldo+siRNA Control.