Mutation	Fragment		Primer 5′-3′
Mut 1	Fragment 1	For	TGAACAATGTG CGCGCC
		Rev HindIII	ATGCCAAGCTTA CTTAGATCGC
	Fragment 2	For XhoI	CCGGGCTC GAGATCCC
		Rev	GGCGCGCACA TTGTTCA
Mut 2	Fragment 1	For	ATTTTGAACA CGGTTCG CGCCTCTTTG GCGCA
		Rev HindIII	ATGCCAAGCTTAC TTAGATCGC
	Fragment 2	For XhoI	CCGGGCTCG AGATCCC
		Rev	AAGAGGCGCG AACCGTG TTCAAAATATG TAGGAGCAGTG
Mut 3	Fragment 1	For	ATTTTGACTGATTGG CCGGCCTCTTTGGCG CATATAAG
		Rev HindIII	ATGCCAAGCTT ACTTAGATCGC
	Fragment 2	For XhoI	CCGGGCTC GAGATCCC
		Rev	AAGAGGCCGGC CAATCAGTCA AAATATGTAG GAGCAGTGCC
Mut 4	Fragment 1	For	TAATTGCAT ACGGTTCT TGTACAACTACTTTC
		Rev HindIII	ATGCCAAGCTTA CTTAGATCGC
	Fragment 2	For XhoI	CCGGGCTC GAGATCCC
		Rev	GTTGTACAA GAACCGTA TGCAATTAGCTTAAG