Table 1.
Protein |
Nucleotide |
Temperature [°C] |
ATPase activity [nmol Pi/min/mg protein] |
Turnover per transporter [min−1] |
% of wildtype |
|
---|---|---|---|---|---|---|
TM287/288 |
wildtype |
ATP - Mg |
50 |
2141 ± 67 |
284 |
- |
25 |
86.1 ± 2.5 |
11.4 |
- |
|||
ATP – Mg +1 mM vanadate |
50 |
165 ± 20 |
21.9 |
7.71 |
||
ATP – Mg +2.5 mM EDTA |
50 |
<0.1 |
<0.01 |
<0.005 |
||
AMP-PNP - Mg |
25 |
<0.1 |
<0.01 |
<0.005 |
||
E517QTM288 |
ATP - Mg |
25 |
0.165 ± 0.015 |
0.0219 |
0.192 |
|
Spin-labeled TM287/288 |
350TM287/475TM288 |
ATP - Mg |
50 |
- |
- |
193* |
460TM287/363TM288 |
ATP - Mg |
50 |
- |
- |
212* |
|
131TM288/248TM288 |
ATP - Mg |
50 |
- |
- |
156* |
|
231TM287/304TM287 |
ATP - Mg |
50 |
- |
- |
146* |
|
150TM287/295TM288 |
ATP - Mg |
50 |
- |
- |
112* |
|
50TM287/271TM287 |
ATP - Mg |
50 |
- |
- |
73* |
|
BmrCD |
wildtype |
ATP - Mg |
25 |
22.9 ± 0.5 |
3.24 |
- |
E592QBmrD |
ATP - Mg |
25 |
0.633 ± 0.053 |
0.0896 |
2.76 |
|
MsbA |
wildtype |
ATP - Mg |
30 |
135 ± 9 |
18.4 |
- |
561MsbA |
ATP - Mg |
30 |
122 ± 17 |
16.6 |
90.4 |
* values are given in respect to an internal wildtype control in each measurement.