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. 2016 Jun 14;7(30):47232–47241. doi: 10.18632/oncotarget.10030

Figure 4. Zingerone does not inhibit the expression of HIF-1α and angiopoietins.

Figure 4

(A–D) Renca cells (A) and SVECs (C) were exposed to zingerone for 24 h and hypoxic conditions for 2 h before harvesting of whole cell extracts. Western blot analysis for HIF-1α (1:1000), VEGF (1:1000), angiopoietin-1 (1:1000), angiopoietin-2 (1:1000), Tie-2 (1:1000) and NOS2 (1:1000) was performed with the respective antibodies. α-tubulin was examined as an internal control. (B and D) Relative protein levels were expressed as the means ± S.D. of three independent experiments. The expression under hypoxic condition was set to 100%.