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. 2016 Jun 18;7(30):47403–47417. doi: 10.18632/oncotarget.10161

Figure 4. Hypersensitivity to rapamycin and mTORC1-addicted growth of other human HCC cell lines.

Figure 4

(A, B) The sensitivities of eight human HCC cell lines to rapamycin (A) and AZD8055 (B) were assessed using a WST assay. Each bar is the average ± SD of triplicate wells. (C) Comparison of the expression and phosphorylation of mTOR and its related signaling molecules among eight human HCC cell lines, including HAK-1A and HAK-1B cells. GAPDH served as loading control. (D) The effect of rapamycin on the phosphorylation of mTOR and S6K in six cell lines. Cells were treated with rapamycin at indicated concentration for 6 hr, and lysates were analyzed using western blotting. In the lower panel, expression levels of p-mTOR (Ser2481) are normalized to the values in the absence of drugs. GAPDH served as loading control. (E) The effect of Raptor knockdown on cell growth. Cells were incubated with a Raptor-siRNA for < 5 days. Each bar is the average ± SD of triplicate wells, *P < 0.05; **P < 0.01 (two-tailed Student t test).