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. Author manuscript; available in PMC: 2017 May 4.
Published in final edited form as: Science. 2016 Nov 4;354(6312):aag1550. doi: 10.1126/science.aag1550

Figure 6. DIG inducible lines exhibit enhanced sensitivity to ABA and salt.

Figure 6

(A–B) ABA-dependent delay of cotyledon greening in 8-day-old seedlings were further amplified upon DEX-mediated induction of DIG1 and DIG2 compared to GFP control quantified by count of green cotyledons (A) and measurement of relative chlorophyll content (B). Error bar reflects the 95% confidence interval around the mean estimate calculated from 3 biological replicates of ca. 50 eight-day-old seedlings each. (C) NaCl-dependent bleaching was observed in 4-week-old plants upon DEX-mediated induction of DIG1 and DIG2. (D-E) DEX-mediated induction of DIG1 (D1) and DIG2 (D2) resulted in more severe inhibition of lateral root growth than GFP (G) control plants on ABA (D) and NaCl (E) plates. (F) DEX-mediated induction of DIG1 and DIG2 led to over-accumulation of pigments in leaves. In D-F, seedlings were transferred to the indicated plates after grown on LS plates for seven days.