Figure 4.

Knocking down neurotransmission of cells specific to adult circuit results in juvenile-like behavior. A, Schematic for transforming adult to L3 behavior by misexpressing TeTx in AWB, AWC, and ASK neurons, and consequently decreasing synaptic transmission from those cells. Chemotaxis is not altered when TeTx is misexpressed individually in AWB*, AWC, or ASK (B–D), but does decline when misexpressed collectively in AWB, AWC, or ASK* (E). F, Adults have a juvenile-like odor preference. Adult worms with decreased synaptic transmission in AWB, AWC, and ASK neurons prefer pyrazine, unlike their wild-type counterparts. Averages and SEM are shown with numbers on each bar representing the number of assays. Data were compared using unpaired, two-tailed t-tests (*p < 0.05) and, when appropriate, with Bonferroni correction for multiple comparisons (B, E). *See also Table 1 for additional tested lines.