Skip to main content
. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Brain Res Bull. 2016 Nov 23;128:48–57. doi: 10.1016/j.brainresbull.2016.10.011

Table 1. Experimental method.

Table 1 summarizes the experimental protocol.

Group ED1* ED 2-6 ED 7-9 ED10*
Saline (control) saline/saline saline washout saline/saline
0.6 mg/kg saline/0.6 0.6 washout saline/0.6
2.5 mg/kg saline/2.5 2.5 washout saline/2.5
10.0 mg/kg saline/10.0 10.0 washout saline/10.0
*

denotes days on which animal behavioral activity and dorsal raphe neuronal activity were recorded for 60 minutes following i.p. saline injection and for an additional 60 minutes following i.p. injection of either saline or 0.6, 2.5, or 10.0 mg/kg methylphenidate (MPD).

On experimental days 2–6 (ED 2–6) animals received only the injection of either saline the experimental dose. On ED 7–9 animals underwent washout and received no injections or recordings. On ED10, a rechallenge saline or MPD dose was administered and behavioral and neuronal recordings resumed following the same protocol as on ED1. All injections were titrated to 0.8ml with 0.9% saline and given in the morning.