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. 2017 Jan 5;27(2):140–153. doi: 10.1093/glycob/cww111

Table II.

Time course GalNAc-T assay monitored by MALDI-TOFa

Enzyme Assay time (h) MUC1-b IgAh MUC7 CARL2 SDC106 SDC3a SDC3b FGF23a FGF23b ANGPTL3 HCPitb HCHIV GMCSF CD59
GalNAc-T1 1 0 (1) 2 (0–1, 3) 1 (0, 2) 0 (1) 0 (1) 0 (1–2) 0 (1) 0 (1) 0 0 0 0 0 0
4 0 (1) 3 (0–2, 4–5) 2 (0–1, 3–4) 0 (1) 0 (1) 0 (1–3) 0 (1) 0 (1) 0 0 0 0 0 0
24 1 (0) 4 (3, 5–6) 4 (1–3, 5) 1 1 (0, 2) 4 (0–3, 5–6) 1 (0, 2–3) 0 = 1 0 0 0 0 0 (1) 0
GalNAc-T13 1 0 (1) 2 (0–1, 3) 2 (0–1, 3) 0 (1) 0 (1) 0 (1–2) 0 (1) 0 (1) 0 0 0 0 0 (1) 0
4 0 = 1 2 = 3 (0–1, 4) 2 (0–1, 3–4) 0 (1) 0 (1) 0 (1–2) 0 (1–2) 0 (1–2) 0 0 0 0 0 (1) 0
24 1 (0, 2) 5 (2–4, 6–7) 4 (1–3, 5–6) 1 (0) 1 (0, 2) 4 (0–3, 5–6) 1 (0, 2) 0 (1–2) 0 0 0 (1) 0 0 (1) 0 (1)
GalNAc-T13-Δ39bpEx9 1 0 0 0 (1) 0 0 0 0 0 0 0 0 0 0 0
4 0 (1) 0 (1) 0 (1) 0 0 0 (1) 0 0 0 0 0 0 0 0
24 0 (1) 0 (1–2) 1 = 2 (0, 3–4) 0 0 0 (1–2) 0 0 (1) 0 0 0 (1) 0 0 (1) 0
GalNAc-T13-Ex10b 1 0 (1) 2 (0–1, 3) 1 (0, 2–3) 1 (0) 0 (1) 0 0 (1) 0 (1) 0 0 0 0 0 (1) 0
4 0 (1) 3 (2, 4–5) 2 (0–1, 3–5) 1 (0) 1 (0) 0 (2, 4) 1 (0, 2) 0 (1–3) 0 0 0 0 0 (1) 0
24 1 (0, 2) 5 (3–4, 6) 5 (3–4, 6) 1 (0) 1 (0, 2) 0 (1–4) 1 (0, 2) 1 (0, 3–4) 0 0 (1) 0 (1) 0 1 (0, 2) 0

aThe numbers inside each cell correspond to the number of GalNAcs incorporated in the major product. The other products, also present in the reaction mixture, are shown in brackets.