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. 1991 Aug 15;88(16):7165–7169. doi: 10.1073/pnas.88.16.7165

Genetic analysis of microtubule motor proteins in Drosophila: a mutation at the ncd locus is a dominant enhancer of nod.

B A Knowles 1, R S Hawley 1
PMCID: PMC52254  PMID: 1908090

Abstract

The nod (no distributive disjunction) and the ncd (non-claret disjunctional) mutations are both female-specific, recessive meiotic mutations in Drosophila melanogaster. Mutations at either locus show high frequencies of nondisjunction at meiosis I and both have been shown to encode kinesin-like proteins. Unlike the ncd mutation, which affects all chromosome pairs, the nod mutation affects only the disjunction of nonexchange chromosomes. Although both the nod and ncd mutations are fully recessive, females doubly heterozygous for nod and ncd mutations show levels of X and fourth chromosome nondisjunction that are 6- to 35-fold above those observed in control females. Exchange between chromosomes can suppress this effect; thus, only nonexchange chromosomes segregating via the distributive system are sensitive in double heterozygotes. Since the phenotype of double heterozygotes mimics that of the nod mutation, we infer that ncd is a dominant enhancer of nod. Failure of ncd to fully complement nod reveals the chromosome segregation machinery to be dosage sensitive. The probability that the distributive system will fail is enhanced in females simultaneously haploinsufficient at the nod and ncd loci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carpenter A. T. A meiotic mutant defective in distributive disjunction in Drosophila melanogaster. Genetics. 1973 Mar;73(3):393–428. doi: 10.1093/genetics/73.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis D. G. Chromosome Behavior under the Influence of Claret-Nondisjunctional in DROSOPHILA MELANOGASTER. Genetics. 1969 Mar;61(3):577–594. doi: 10.1093/genetics/61.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Endow S. A., Henikoff S., Soler-Niedziela L. Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin. Nature. 1990 May 3;345(6270):81–83. doi: 10.1038/345081a0. [DOI] [PubMed] [Google Scholar]
  4. GRELL R. F. A new model for secondary nondisjunction: the role of distributive pairing. Genetics. 1962 Dec;47:1737–1754. doi: 10.1093/genetics/47.12.1737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hays T. S., Deuring R., Robertson B., Prout M., Fuller M. T. Interacting proteins identified by genetic interactions: a missense mutation in alpha-tubulin fails to complement alleles of the testis-specific beta-tubulin gene of Drosophila melanogaster. Mol Cell Biol. 1989 Mar;9(3):875–884. doi: 10.1128/mcb.9.3.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kimble M., Church K. Meiosis and early cleavage in Drosophila melanogaster eggs: effects of the claret-non-disjunctional mutation. J Cell Sci. 1983 Jul;62:301–318. doi: 10.1242/jcs.62.1.301. [DOI] [PubMed] [Google Scholar]
  7. McDonald H. B., Goldstein L. S. Identification and characterization of a gene encoding a kinesin-like protein in Drosophila. Cell. 1990 Jun 15;61(6):991–1000. doi: 10.1016/0092-8674(90)90064-l. [DOI] [PubMed] [Google Scholar]
  8. McDonald H. B., Stewart R. J., Goldstein L. S. The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor. Cell. 1990 Dec 21;63(6):1159–1165. doi: 10.1016/0092-8674(90)90412-8. [DOI] [PubMed] [Google Scholar]
  9. Nicklas R. B. Chromosome segregation mechanisms. Genetics. 1974 Sep;78(1):205–213. doi: 10.1093/genetics/78.1.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nicklas R. B., Staehly C. A. Chromosome micromanipulation. I. The mechanics of chromosome attachment to the spindle. Chromosoma. 1967;21(1):1–16. doi: 10.1007/BF00330544. [DOI] [PubMed] [Google Scholar]
  11. Sequeira W., Nelson C. R., Szauter P. Genetic analysis of the claret locus of Drosophila melanogaster. Genetics. 1989 Nov;123(3):511–524. doi: 10.1093/genetics/123.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Walker R. A., Salmon E. D., Endow S. A. The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature. 1990 Oct 25;347(6295):780–782. doi: 10.1038/347780a0. [DOI] [PubMed] [Google Scholar]
  13. Yamamoto A. H., Komma D. J., Shaffer C. D., Pirrotta V., Endow S. A. The claret locus in Drosophila encodes products required for eyecolor and for meiotic chromosome segregation. EMBO J. 1989 Dec 1;8(12):3543–3552. doi: 10.1002/j.1460-2075.1989.tb08526.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zhang P., Hawley R. S. The genetic analysis of distributive segregation in Drosophila melanogaster. II. Further genetic analysis of the nod locus. Genetics. 1990 May;125(1):115–127. doi: 10.1093/genetics/125.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zhang P., Knowles B. A., Goldstein L. S., Hawley R. S. A kinesin-like protein required for distributive chromosome segregation in Drosophila. Cell. 1990 Sep 21;62(6):1053–1062. doi: 10.1016/0092-8674(90)90383-p. [DOI] [PubMed] [Google Scholar]
  16. Zitron A. E., Hawley R. S. The genetic analysis of distributive segregation in Drosophila melanogaster. I. Isolation and characterization of Aberrant X segregation (Axs), a mutation defective in chromosome partner choice. Genetics. 1989 Aug;122(4):801–821. doi: 10.1093/genetics/122.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]

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