Figure 5. Effects of androgen on NF-κB expression in bladder cancer cells.

Western blotting of AR, NF-κB, and p-NF-κB in 647V-AR, 5637-AR, and UMUC3 cells. Total protein extracted from each line treated with ethanol (mock), 10 nM R1881, and/or 5 μM HF for 48 hours was immunoblotted for AR (110 kDa), NF-κB (65 kDa), or p-NF-κB (65 kDa). GAPDH (37 kDa) served as an internal control.