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. 2016 Jun 7;7(31):49349–49367. doi: 10.18632/oncotarget.9885

Figure 5. CCL2 gene silencing in breast cancer cells by Ca-TAT/siRNA complexes inhibits cancer stem cell renewal.

Figure 5

A-B. MDA-MB-231 breast tumor xenografts (N=6 animals per group) were treated with control (Con) or Ca-TAT complexed to CCL2 siRNAs (huCCL2si1, huCCL2si2), and were analyzed for CD24 and CD44 expression by flow cytometry (A), or ALDH1 expression by immunohistochemistry stain (B). ALDH1 expression was quantified by Image J; arbitrary units are shown. Scale bar=100 microns. C. MDA-MB-231 cells in culture were transfected with Ca-TAT/siRNA complexes and examined for mammosphere formation. Representative images are shown at passage 4. Scale bar=200 microns. Statistical analysis was performed by One Way ANOVA followed by Bonferonni post-hoc comparisons with Control group. Statistical significance was determined by p-value less than 0.05. *p<0.05, **p<0.01. Mean+SEM is shown.