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. 2016 Jul 6;7(31):49588–49596. doi: 10.18632/oncotarget.10412

Figure 4. TPL induces cell apoptosis and M/G2 phase arrest.

Figure 4

(A) Assay of cell apoptosis by Hoechst 33258. Both CNE2 (C2) and CNE2-SR (C2-SR) cells were cultured in 6-well culture plates and treated with 6 Gy X-ray and 25 nM TPL for 48 h. (B). Statistical analysis cell apoptosis based on Hoechst 33258 staining from three independent experiments (n = 3). The data are presented as mean ± standard deviation (SD). *p < 0.05. (C) Measurement of cell apoptosis by flow cytometry after Annexin V-FITC/propidium iodide (PI). (D) Cell cycle analysis of CNE2 and CNE2-SR cells after 6 Gy X-ray and/or 25 nM TPL for 48 h.