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. 1991 Sep 1;88(17):7600–7604. doi: 10.1073/pnas.88.17.7600

Depolarization-induced changes in cellular energy production.

M Erecińska 1, D Nelson 1, B Chance 1
PMCID: PMC52349  PMID: 1715571

Abstract

Addition of high concentrations of KC1 to preparations of rat brain synaptosomes incubated with either glucose or pyruvate caused a transient stimulation of oxygen uptake. This increased respiration was insensitive to 1 mM ouabain and 10 microM ruthenium red but was dependent upon the presence of calcium. With 40 mM KCl in the incubation medium, the levels of high-energy phosphate compounds in the synaptosomes were unaltered, whereas pyridine nucleotides underwent a rapid, albeit small and temporary, oxidation. It is postulated that there is a calcium-dependent mechanism in synaptosomes through which the function of the mitochondrial respiratory chain or of oxidative phosphorylation is stimulated directly without the involvement of either adenine nucleotides or mitochondrial dehydrogenases.

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Selected References

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