Skip to main content

. 1994 Jun 25;22(12):2423. doi: 10.1093/nar/22.12.2423

Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments.

G L Costa ¹, M P Weiner ¹

PMCID: PMC523705 PMID: 8036174

2423

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Clark J. M., Joyce C. M., Beardsley G. P. Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli. J Mol Biol. 1987 Nov 5;198(1):123–127. doi: 10.1016/0022-2836(87)90462-1. [DOI] [PubMed] [Google Scholar]
Clark J. M. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 1988 Oct 25;16(20):9677–9686. doi: 10.1093/nar/16.20.9677. [DOI] [PMC free article] [PubMed] [Google Scholar]
Hu G. DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3' end of a DNA fragment. DNA Cell Biol. 1993 Oct;12(8):763–770. doi: 10.1089/dna.1993.12.763. [DOI] [PubMed] [Google Scholar]
Stoker A. W. Cloning of PCR products after defined cohesive termini are created with T4 DNA polymerase. Nucleic Acids Res. 1990 Jul 25;18(14):4290–4290. doi: 10.1093/nar/18.14.4290. [DOI] [PMC free article] [PubMed] [Google Scholar]

ACTIONS

RESOURCES