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. 1994 Aug 25;22(16):3312–3316. doi: 10.1093/nar/22.16.3312

Human xeroderma pigmentosum group G gene encodes a DNA endonuclease.

Y Habraken 1, P Sung 1, L Prakash 1, S Prakash 1
PMCID: PMC523723  PMID: 8078765

Abstract

Because of defective nucleotide excision repair of ultraviolet damaged DNA, xeroderma pigmentosum (XP) patients suffer from a high incidence of skin cancers. Cell fusion studies have identified seven XP complementation groups, A to G. Previous studies have implicated the products of these seven XP genes in the recognition of ultraviolet-induced DNA damage and in incision of the damage-containing DNA strand. Here, we express the XPG-encoded protein in Sf9 insect cells and purify it to homogeneity. We demonstrate that XPG is a single-strand specific DNA endonuclease, thus identifying the catalytic role of the protein in nucleotide excision repair. We suggest that XPG nuclease acts on the single-stranded region created as a result of the combined action of the XPB helicase and XPD helicase at the DNA damage site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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