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. 1994 Aug 25;22(16):3406–3411. doi: 10.1093/nar/22.16.3406

Characterization of four human YAC libraries for clone size, chimerism and X chromosome sequence representation.

R Nagaraja 1, J Kere 1, S MacMillan 1, M J Masisi 1, D Johnson 1, B J Molini 1, G R Halley 1, K Wein 1, M Trusgnich 1, B Eble 1
PMCID: PMC523736  PMID: 8078777

Abstract

Four collections of human X-specific YACs, derived from human cells containing supernumerary X chromosomes or from somatic cell hybrids containing only X human DNA were characterized. In each collection, 80-85% of YAC strains contained a single X YAC. Five thousand YACs from the various libraries were sized, and cocloning was assessed in subsets by the fraction of YAC insert-ends with non-X sequences. Cocloning was substantial, ranging up to 50% for different collections; and in agreement with previous indications, in all libraries the larger the YACs, the higher the level of cocloning. In libraries made from human-hamster hybrid cells, expected numbers of clones were recovered by STS-based screening; but unexpectedly, the two collections from cells with 4 or 5 X chromosomes yielded numbers of YACs corresponding to an apparent content of only about two X equivalents. Thus it is possible that the DNA of inactive X chromosomes is poorly cloned into YACs, speculatively perhaps because of its specialized chromatin structure.

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