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. 1994 Nov 25;22(23):4869–4871. doi: 10.1093/nar/22.23.4869

Human and E.coli excinucleases are affected differently by the sequence context of acetylaminofluorene-guanine adduct.

D Mu 1, E Bertrand-Burggraf 1, J C Huang 1, R P Fuchs 1, A Sancar 1, B P Fuchs 1
PMCID: PMC523749  PMID: 7702657

Abstract

Synthetic DNA substrates containing an acetylaminofluorene (AAF) adduct at each of the three guanine in the G1G2CG3CC sequence were constructed and tested as substrates for reconstituted E.coli (A)BC excinuclease and human excinuclease in HeLa cell-free extract (CFE). The (A)BC excinulcease repaired the three substrates with relative efficiencies of G1:G2:G3 of 100:18:66 in agreement with an earlier report [Seeberg, E., and Fuchs, R.P.P. (1990) Proc. Natl Acad. Sci. USA 87, 191-194]. The same lesions were repaired by the human excinuclease with the strikingly different efficiencies of G1:G2:G3 as 38:100:68. These results reveal that the human excinuclease is affected by the sequence context of the lesion in a different manner than its prokaryotic counterpart.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertrand-Burggraf E., Kemper B., Fuchs R. P. Endonuclease VII of phage T4 nicks N-2-acetylaminofluorene-induced DNA structures in vitro. Mutat Res. 1994 May;314(3):287–295. doi: 10.1016/0921-8777(94)90072-8. [DOI] [PubMed] [Google Scholar]
  2. Burnouf D., Koehl P., Fuchs R. P. Single adduct mutagenesis: strong effect of the position of a single acetylaminofluorene adduct within a mutation hot spot. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4147–4151. doi: 10.1073/pnas.86.11.4147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fuchs R. P. DNA binding spectrum of the carcinogen N-acetoxy-N-2-acetylaminofluorene significantly differs from the mutation spectrum. J Mol Biol. 1984 Jul 25;177(1):173–180. doi: 10.1016/0022-2836(84)90063-9. [DOI] [PubMed] [Google Scholar]
  4. Huang J. C., Svoboda D. L., Reardon J. T., Sancar A. Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3' to the photodimer. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3664–3668. doi: 10.1073/pnas.89.8.3664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones B. K., Yeung A. T. DNA base composition determines the specificity of UvrABC endonuclease incision of a psoralen cross-link. J Biol Chem. 1990 Feb 25;265(6):3489–3496. [PubMed] [Google Scholar]
  6. Koehl P., Burnouf D., Fuchs R. P. Construction of plasmids containing a unique acetylaminofluorene adduct located within a mutation hot spot. A new probe for frameshift mutagenesis. J Mol Biol. 1989 May 20;207(2):355–364. doi: 10.1016/0022-2836(89)90259-3. [DOI] [PubMed] [Google Scholar]
  7. Koffel-Schwartz N., Verdier J. M., Bichara M., Freund A. M., Daune M. P., Fuchs R. P. Carcinogen-induced mutation spectrum in wild-type, uvrA and umuC strains of Escherichia coli. Strain specificity and mutation-prone sequences. J Mol Biol. 1984 Jul 25;177(1):33–51. doi: 10.1016/0022-2836(84)90056-1. [DOI] [PubMed] [Google Scholar]
  8. Manley J. L., Fire A., Cano A., Sharp P. A., Gefter M. L. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855–3859. doi: 10.1073/pnas.77.7.3855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sancar A., Rupp W. D. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell. 1983 May;33(1):249–260. doi: 10.1016/0092-8674(83)90354-9. [DOI] [PubMed] [Google Scholar]
  10. Seeberg E., Fuchs R. P. Acetylaminofluorene bound to different guanines of the sequence -GGCGCC- is excised with different efficiencies by the UvrABC excision nuclease in a pattern not correlated to the potency of mutation induction. Proc Natl Acad Sci U S A. 1990 Jan;87(1):191–194. doi: 10.1073/pnas.87.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Svoboda D. L., Smith C. A., Taylor J. S., Sancar A. Effect of sequence, adduct type, and opposing lesions on the binding and repair of ultraviolet photodamage by DNA photolyase and (A)BC excinuclease. J Biol Chem. 1993 May 15;268(14):10694–10700. [PubMed] [Google Scholar]
  12. Thomas D. C., Husain I., Chaney S. G., Panigrahi G. B., Walker I. G. Sequence effect on incision by (A)BC excinuclease of 4NQO adducts and UV photoproducts. Nucleic Acids Res. 1991 Jan 25;19(2):365–370. doi: 10.1093/nar/19.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thomas D. C., Veaute X., Kunkel T. A., Fuchs R. P. Mutagenic replication in human cell extracts of DNA containing site-specific N-2-acetylaminofluorene adducts. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7752–7756. doi: 10.1073/pnas.91.16.7752. [DOI] [PMC free article] [PubMed] [Google Scholar]

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