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. 1994 Nov 25;22(23):4983–4988. doi: 10.1093/nar/22.23.4983

The pseudodisaccharides: a novel class of group I intron splicing inhibitors.

J Rogers 1, J Davies 1
PMCID: PMC523767  PMID: 7800490

Abstract

Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibited the self-splicing of the sunY intron of phage T4 with a Ki of 8.5 microM (+/- 5 microM) and was active against other group I introns. Inhibition was found to be competitive with the substrate guanosine, unlike aminoglycoside antibiotics, which act non-competitively to inhibit the splicing of group I introns. Competitive inhibitors of group I intron splicing known to date all contain a guanidino group that was thought to be required for inhibition; lysinomicin lacks a guanidino group.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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