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. 1994 Nov 25;22(23):5076–5078. doi: 10.1093/nar/22.23.5076

Selective chemical autoligation on a double-stranded DNA template.

M K Herrlein 1, R L Letsinger 1
PMCID: PMC523780  PMID: 7800502

Abstract

We show that a double-stranded DNA segment serves as an effective template for spontaneously coupling short pyrimidine oligonucleotides containing terminal -P(O)(O-)S- and BrCH2C(O)NH- groups. The efficiency of this autoligation depends markedly on proper base-pairing between the probe oligomers and the double-stranded target. This chemistry should be useful in designing highly selective probes for double-stranded polynucleotide segments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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