Figure 6.

Effect of treatment on proliferation of renal tumor cells in Tsc2^+/− mice.

Kidney sections were prepared from 13-month-old Tsc2^+/− mice after treatment and stained with antibody against Ki67 by IHC to assess proliferation of tumor cells. (A) Expression of Ki67 in renal tumors. Representative sections were presented to show expression of Ki67 in tumor cells after 2-month treatment with vehicle, everolimus, sorafenib, and everolimus plus sorafenib. Scale bars are 100.1 μm. (B) Proliferation of tumor cells. Twenty-five lesions randomly chosen from each group after 2-month treatment were used to detect Ki67-positive tumor cells using ImageJ. Everolimus alone or everolimus plus sorafenib significantly inhibited proliferation of tumor cells. Sorafenib alone significantly inhibited proliferation of tumor cells but to a smaller extent.