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. 2016 Jul 18;7(32):50845–50863. doi: 10.18632/oncotarget.10689

Figure 6. PE8 extends yeast CLS by attenuating the inhibitory effect of PKA on SNF1.

Figure 6

A. Cells of the wild-type (WT) and indicated mutant strains were grown in the synthetic minimal YNB medium (0.67% Yeast Nitrogen Base without amino acids) initially containing 2% glucose, in the presence of 0.3% PE8 (ethanol was used as a vehicle at the final concentration of 1.5%) or in its absence (cells were subjected to ethanol-mock treatment). Survival curves of chronologically aging WT and mutant strains cultured with or without 0.3% PE8 are shown. Data are presented as means ± SEM (n = 6). The dotted line indicates the predicted survival curve of a particular mutant strain cultured with PE8 if this PE exhibits an additive longevity-extending effect with the mutation. Data for the mock-treated WT strain and for the WT strain cultured with PE8 are replicated in all graphs of this Figure. B. The effect of PE8 on the signaling pathways and protein kinases integrated into the longevity-defining network. This effect is inferred from the data presented in A., Tables 2 and 3, and Suppl. Figs. S4 and S10. Abbreviations: as in the legend to Figure 1.