Figure 3. In situ hybridization of Wnt pathway-related genes in SP600125-treated and untreated larvae at 24 h after neomycin treatment.
Expression of axin2, ctnnb1, ctnnb2, and tcf7l2 is significantly decreased in SP600125-treated neuromasts during the regeneration period. (axin2: n = 28 neuromasts from control animals, n = 20 neuromasts from SP600125-treated larvae; ctnnb1: n = 20 neuromasts from control animals, n = 16 neuromasts from SP600125-treated larvae; ctnnb2: n = 18 neuromasts from control animals, n = 16 neuromasts from SP600125-treated larvae; tcf7l2: n = 22 neuromasts from control animals, n = 20 neuromasts from SP600125-treated larvae). Results from one representative neuromast are shown. Scale bar = 10 μm.