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. 2016 Jul 8;7(32):52032–52044. doi: 10.18632/oncotarget.10496

Figure 3. sTLR4/MD-2 complex inhibits the cell cycle, migration and invasion of CRC cells induced by LPS.

Figure 3

SW480 cells were incubated with PBS, LPS (1.0 μg/ml), sTLR4 (LPS 1.0 μg/ml + sTLR4 5.0 μg/ml), MD-2 (LPS 1.0 μg/ml + MD-2 1.25 μg/ml), sTLR4/MD-2 (LPS 1.0 μg/ml + sTLR4/MD-2 6.25 μg/ml) for 24 hours (the cell cycle and invasion assay) or 48 hours (the cell migration assay), respectively. A. Cell cycle analysis (upper), quantitative results (bottom). B. Transwell Matrigel invasion assay of SW480 cells was analyzed. ×100 magnification. C. Wound migration assay of SW480 cells (left) and quantitative results (right). ×400 magnification. *P < 0.05, data were analyzed by One-way ANOVA test with Graphpad Prism 5. Representative images of three independent experiments were shown.