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. 2016 Jul 13;7(32):52317–52328. doi: 10.18632/oncotarget.10562

Figure 5. VDAC1 and HPV16 E7 protein levels were impacted in S12 and SiHa cell lines transfected with the corresponding CRISPR-VDAC1 or CRISPR-HPV16 E7 and overexpression plasmids.

Figure 5

Western blots are shown for A. VDAC1 protein expression in S12 cells transfected with CRISPR-VDAC1 and an ectopic VDAC1 plasmid. B. HPV16 E7 expression in S12 cells transfected with CRISPR-HPV16 E7 and an ectopic HPV16 E7 plasmid. C. VDAC1 expression in SiHa cells transfected with CRISPR-VDAC1 and an ectopic VDAC1 plasmid and D. HPV16 E7 expression in SiHa cells transfected with CRISPR-HPV16 E7 and an ectopic HPV16 E7 plasmid. Western blots are shown for E. VDAC1 and HPV16 E7 expression in S12 cells co-transfected with CRISPR-VDAC1 and an ectopic HPV16 E7 plasmid. F. VDAC1 and HPV16 E7 expression in S12 cells co-transfected with CRISPR-HPV16 E7 and an ectopic VDAC1 plasmid. G. VDAC1 and HPV16 E7 expression in SiHa cells co-transfected with CRISPR-VDAC1 and an ectopic HPV16 E7 plasmid and H. VDAC1 and HPV16 E7 expression in SiHa cells co-transfected with CRISPR-HPV 16 E7 and an ectopic VDAC1 plasmid. For all panels, quantitative measurements are shown above the Western blots. The expression of GAPDH was used for normalization. All data are presented as the mean ± SD of three independent experiments, * P < 0.05.