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. 1991 Sep 1;88(17):7854–7858. doi: 10.1073/pnas.88.17.7854

Mechanism of phenolic activation of Agrobacterium virulence genes: development of a specific inhibitor of bacterial sensor/response systems.

K M Hess 1, M W Dudley 1, D G Lynn 1, R D Joerger 1, A N Binns 1
PMCID: PMC52402  PMID: 1909032

Abstract

The aglycone of the dihydrodiconiferyl alcohol glycosides, a series of phenolic growth factors able to substitute for some of the hormone requirements of tobacco cell division, are also potent inducers of virulence gene expression in Agrobacterium tumefaciens. However, these factors do not conform to the previously established structural requirements necessary for vir expression. Systematic evaluation of the structural requirements of these inducers has led to a model detailing the role of the phenolics in induction. With this model, a specific inhibitor of vir induction has been developed. This inhibitor does not affect the induction of other genes on the Ti plasmid but irreversibly blocks vir expression. The inhibitor has been used to show that the inducing phenolics must be constantly present to maintain expression of the vir regulon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAUN A. C. Conditioning of the host cell as a factor in the transformation process in crown gall. Growth. 1952 Jun;16(2):65–74. [PubMed] [Google Scholar]
  2. BRAUN A. C., MANDLE R. J. Studies on the inactivation of the tumor-inducing principle in crown gall. Growth. 1948 Dec;12(4):255–269. [PubMed] [Google Scholar]
  3. Cangelosi G. A., Ankenbauer R. G., Nester E. W. Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6708–6712. doi: 10.1073/pnas.87.17.6708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coulson A. F., Knowles J. R., Priddle J. D., Offord R. E. Uniquely labelled active site sequence in chicken muscle triose phosphate isomerase. Nature. 1970 Jul 11;227(5254):180–181. doi: 10.1038/227180a0. [DOI] [PubMed] [Google Scholar]
  5. Huang Y., Morel P., Powell B., Kado C. I. VirA, a coregulator of Ti-specified virulence genes, is phosphorylated in vitro. J Bacteriol. 1990 Feb;172(2):1142–1144. doi: 10.1128/jb.172.2.1142-1144.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jin S. G., Prusti R. K., Roitsch T., Ankenbauer R. G., Nester E. W. Phosphorylation of the VirG protein of Agrobacterium tumefaciens by the autophosphorylated VirA protein: essential role in biological activity of VirG. J Bacteriol. 1990 Sep;172(9):4945–4950. doi: 10.1128/jb.172.9.4945-4950.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jin S., Roitsch T., Ankenbauer R. G., Gordon M. P., Nester E. W. The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation. J Bacteriol. 1990 Feb;172(2):525–530. doi: 10.1128/jb.172.2.525-530.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lynn D. G., Chen R. H., Manning K. S., Wood H. N. The structural characterization of endogenous factors from Vinca rosea crown gall tumors that promote cell division of tobacco cells. Proc Natl Acad Sci U S A. 1987 Feb;84(3):615–619. doi: 10.1073/pnas.84.3.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Melchers L. S., Regensburg-Tuïnk A. J., Schilperoort R. A., Hooykaas P. J. Specificity of signal molecules in the activation of Agrobacterium virulence gene expression. Mol Microbiol. 1989 Jul;3(7):969–977. doi: 10.1111/j.1365-2958.1989.tb00246.x. [DOI] [PubMed] [Google Scholar]
  10. Pazour G. J., Das A. virG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein. J Bacteriol. 1990 Mar;172(3):1241–1249. doi: 10.1128/jb.172.3.1241-1249.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Saier M. H., Jr, Wu L. F., Reizer J. Regulation of bacterial physiological processes by three types of protein phosphorylating systems. Trends Biochem Sci. 1990 Oct;15(10):391–395. doi: 10.1016/0968-0004(90)90238-7. [DOI] [PubMed] [Google Scholar]
  12. Shimoda N., Toyoda-Yamamoto A., Nagamine J., Usami S., Katayama M., Sakagami Y., Machida Y. Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6684–6688. doi: 10.1073/pnas.87.17.6684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith C. E., Dudley M. W., Lynn D. G. Vegetative/Parasitic transition: control and plasticity in striga development. Plant Physiol. 1990 May;93(1):208–215. doi: 10.1104/pp.93.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stachel S. E., An G., Flores C., Nester E. W. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 1985 Apr;4(4):891–898. doi: 10.1002/j.1460-2075.1985.tb03715.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stachel S. E., Nester E. W. The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens. EMBO J. 1986 Jul;5(7):1445–1454. doi: 10.1002/j.1460-2075.1986.tb04381.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stock J. B., Stock A. M., Mottonen J. M. Signal transduction in bacteria. Nature. 1990 Mar 29;344(6265):395–400. doi: 10.1038/344395a0. [DOI] [PubMed] [Google Scholar]
  17. Winans S. C., Kerstetter R. A., Nester E. W. Transcriptional regulation of the virA and virG genes of Agrobacterium tumefaciens. J Bacteriol. 1988 Sep;170(9):4047–4054. doi: 10.1128/jb.170.9.4047-4054.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winans S. C. Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolic compounds, phosphate starvation, and acidic growth media. J Bacteriol. 1990 May;172(5):2433–2438. doi: 10.1128/jb.172.5.2433-2438.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zambryski P., Tempe J., Schell J. Transfer and function of T-DNA genes from agrobacterium Ti and Ri plasmids in plants. Cell. 1989 Jan 27;56(2):193–201. doi: 10.1016/0092-8674(89)90892-1. [DOI] [PubMed] [Google Scholar]

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