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Stability profile of T4L mutants
| ΔGunf (kcal mol−1)* | |||
|---|---|---|---|
| T4L-construct | 23 °C (± s.d.) | 30 °C (± s.d.) | 37 °C (± s.d.) |
| WT | 14.3 (1.5) | ||
| D70N | 9.2 (0.6) | 7.8 (0.7) | 6.8 (0.4) |
| L99A | 7.9 (0.7) | 6.5 (0.3) | 5.3 (0.7) |
| L99A/A130S | 6.6 (0.5) | 4.7 (0.3) | 3.5 (0.3) |
determined at pH 7.2
