Table 1.

Activities and stereoselectivities of P450 variants for the reaction of styrene with ethyl diazoacetate.

Catalyst	Yield	TTN[a]	dr [cis:trans]	ee cis [%][b]	ee trans [%][c]
hemin	16	79	13:87	−2	−4
P450BM3	1	7	12:88	0	−2
P450BM3-T268A	67	338	1:99	−18	−97
P450BM3-CIS-T438S	62	311	93:7	−97	−79
P450BioI	27	135	12:88	8	13
P450BioI-T238A	48	241	71:29	95	−24
P450cam	41	207	88:12	−43	9
P450cam-T252A	30	151	71:29	−86	−5
P450eryF (A245)	70	349	89:11	−99	−19
CYP142	49	246	44:56	−84	−6
CYP142-T234A	54	272	90:10	−97	−14
CYP164A2	7	34	14:85	5	2
CYP164A2-T260A	70	350	18:82	−82	−9
CYP107N1	7	36	9:91	0	−4
CYP107N1-T251A	48	238	9:91	−3	−3
P450nor	5	27	12:88	−6	−3
P450nor-T243A	13	66	10:90	−3	−2
P450EpoK	49	247	11:89	−15	−22
P450EpoK-T258A	44	219	18:82	−25	−14
P450PikC	50	249	8:92	−2	3
P450PikC-T247A	46	231	7:93	−15	32
P450RhF	52	258	9:91	−3	−2
P450RhF-T275A	34	171	12:88	−11	−2
P450TxtE	37	187	10:90	6	−2
P450TxtE-T250A	45	225	10:90	−2	−2
P450TylH1	48	242	13:87	16	8
P450TylH1-T279A	50	251	10:90	1	−28

^[a]TTN =total turnover number.

^[b](1R,2S)–(1S,2R).

^[c](1R,2R)–(1S,2S).

TTNs and stereoselectivities were determined by chiral GC analysis.