Figure 2. dmsr-1 mutations suppress flp-13 induced quiescence.
(A) Fraction of animals quiescent for pharyngeal pumping two hours after induction of flp-13 overexpression by exposure to a 30 min 33°C heat pulse. Statistical significance was assessed using a Fisher’s exact test. N = 15–20 for each genotype. Asterisks indicate significant difference (p<0.0001) compared to wild type animals two hours after heat pulse. (B) Rate of pharyngeal pumping in wild type or dmsr-1(qn45) mutants with or without the Phsp16.2:flp-13 transgene comparing the behavior pre-heat pulse to two hours post-heat pulse. (C) Body movement quiescence in a two hour period starting two hours after induction of flp-13 overexpression. (D) Total body movement activity in the same two-hour period as (C). Activity is the sum of pixels changed between sequential images. Statistical significance in panels B-D was assessed using a 2-Way ANOVA with post-hoc pairwise comparisons made using Bonferroni correction method. Error bars denote Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001.