Video 3. The order of delamination foreshadows the mediolateral gradient of neuroblasts differentiation within the SAG.
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DOI: 10.7554/eLife.22268.012
A cohort of 144 delaminated neuroblasts was analyzed for time of delamination. Reconstructed cell centers were color-coded according to four delamination intervals: 18–20 hpf white, 20–22 hpf yellow, 22–24 hpf orange, 24–30 hpf red. Note that neuroblasts exit randomly from the delamination domain. Those delaminating earlier are located more medially in the SAG than the later delaminating ones prefiguring the gradient of differentiation. Reconstructed cell centers were displayed as colored dots together with the corresponding volume rendering images (plasma membranes in grey level) with higher intensity on the left hand side. Tg[cldnb:lynGFP] Tg[Isl3:GFP] embryo was injected with H2B-mCherry mRNA at 1cell-stage. ID Dataset: 140426aX.