Abstract
Hepatocarcinogenesis is a prerequisite to hepatocellular carcinoma (HCC), which is one of the most common cancers among humans. Therefore, it is important to search for agents that protect against hepatocarcinogenesis. The present study aimed to investigate the protective effects of a combination of taurine and curcumin against experimental hepatocarcinogenesis induced by diethyl nitrosamine (DENA) in a rat model. A total of 100 rats were divided into eight groups. Eight weeks following DENA injection and treatment with curcumin and taurine, the rats were sacrificed to obtain blood and hepatic tissue samples for the evaluation of various markers and histopathological observations. Serum levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), α-fetoprotein (AFP) and α-L-fucosidase (AFU) were determined. Rats injected with DENA for eight weeks showed a high percentage of malignant changes in hepatic tissues, as well as a significant increases in the serum levels of AFP and AFU and significant reductions in the serum levels of IL-2 and IFN-γ. Treatment with curcumin and taurine markedly reduced the extent of malignant changes in the rat liver tissues, with their liver tissues showing patterns similar to that of the normal control rats. In addition, this combination resulted in normal serum levels of IL-2, IFN-γ, AFP and AFU. The results of the present study suggested that a combination of curcumin and taurine may be a novel prophylactic agent against hepatocarcinogenesis in high-risk groups exposed to chemical hepatocarcinogens.
Keywords: rat hepatocarcinogenesis, curcumin, taurine, interleukin-2, interferon-γ, α-fetoprotein, α-L-fucosidase
Introduction
It has previously been demonstrated that treatment of rats with diethylnitroseamine (DENA) induced moderate and well-differentiated forms of hepatocellular carcinoma (HCC) that resembled human HCC (1) HCC is the second most common cause of cancer-associated mortality in males and the sixth among females (2). In previous studies, a diet rich in dietary antioxidants and phytochemicals was shown to decrease the risk of HCC (3–5). Therefore, identifying potential chemopreventive agents in the human diet, and their underlying molecular mechanisms, has been considered the best strategy for protecting against human hepatocarcinogenesis (3). Numerous investigators have attempted to use naturally occurring compounds for preventing or inhibiting carcinogenesis (6–10). Furthermore, various natural bioactive compounds have been shown to exert anti-cancer effects, including reversing the epigenetic mechanisms underlying cancer, reducing tumorigenesis and metastasis, and targeting cancer stem cells (11).
Previous studies have demonstrated that taurine, which is an amino-acid derivative, exhibits a protective effect against oxidation-induced cellular stress by acting as a free radical scavenger in various cells and tissues (12–15). In addition, taurine has been shown to exert hepatoprotective effects, including inhibiting the accumulation of extracellular matrix components in an experimental model of liver fibrosis (16,17). Furthermore, it was shown to protect against genotoxicity induced by anti-neoplastic drugs in somatic and germ tissues, and may be of therapeutic potential in alleviating the risk of secondary tumors following chemotherapy (18,19). Taurine also reduced lipid peroxidation and the levels of proinflammatory cytokines in a rat model of acute pancreatitis by increasing the levels of superoxide dismutase and glutathione peroxidase (20). In addition, taurine has been shown to exert an anti-cancer effect by inducing the apoptosis of cancer cells, although the underlying molecular mechanism of this effect is not well understood (21).
In a previous study, p53-upregulated modulator of apoptosis (PUMA) was shown to respond to taurine treatment in human colorectal cancer cells, which may be useful in the targeted therapy of p53-deficient colorectal cancer (22). Furthermore, it has been demonstrated that silencing the PUMA gene with specific small interfering RNA (siRNA) was able to significantly reduce the ability of taurine to inhibit the proliferation and induce the apoptosis of human HCC HepG2 cells (23).
Curcumin (diferuloyl methane), which is a yellow curry pigment obtained from turmeric extract (Curcuma longa), has been shown to be a potent anti-inflammatory, anti-carcinogenic and anti-oxidative agent in a number of pre-clinical trials (24–27). In addition, curcumin was demonstrated to exert chemopreventive effects against DENA-induced murine hepatocarcinogenesis (28). The inhibition of tumor formation by curcumin has been attributed to its anti-initiation and anti-promotional effects. The former effect may result from its ability to inhibit the formation of DNA damage (29–31), whereas the latter may be mediated via anti-proliferation effects or induction of apoptosis in the initiated cells (32–35).
Curcumin has previously been shown to be a potent immunomodulatory agent that can modulate the activation of T-cells, B-cells, macrophages, neutrophils, natural killer cells and dendritic cells (36). Furthermore, curcumin was shown to downregulate the expression of various proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1, IL-2, IL-6, IL-8 and IL-12, by inactivation of the transcription factor nuclear factor-κB (37).
In another study, curcumin and taurine alone or in combination inhibited the growth of leukemic cells propagated in vitro, and increased the level of interferon-γ (IFN-γ) in the conditioned media of the cells (38). Furthermore, curcumin and taurine treatment resulted in a significant increase in CD25+ mononuclear leukocytes in healthy control and cirrhotic patients, and when propagated in vitro, although the increase was not significant in HCC patients (39).
The present study aimed to investigate the protective effects of a combination of taurine and curcumin in a rat model of DENA-induced hepatocarcinogenesis.
Materials and methods
Animals
A total of 100 male albino rats, weighing 80–100 g and aged 6–8 weeks, were obtained from the Institute of Ophthalmology (Giza, Egypt). The rats were housed five per cage under optimal conditions of temperature (27–30°C) and humidity (40–70%), and were fed a standard pellet diet and water. The diet consisted of ~20% protein, 5% fiber, 3.5% fat and 6.5% ash, as well as vitamin mixtures. Feed and water were available ad libitum throughout the experimental period. Ethical approval for the present study was granted by the Institutional Animal Care and Use Committee of Cairo University (Cairo, Egypt).
Experimental procedure
A suspension of curcumin in saline was prepared at a concentration of 400 mg/40 ml, after which 2.5 ml curcumin suspension was orally administered to a rat (100 mg/kg body weight). Taurine in saline was prepared at a concentration of 1,000 mg/100 ml, after which 1 ml taurine solution was administered orally to a rat (100 mg/kg body weight).
Rats were divided into eight groups, as follows: i) Control group (n=10), in which the rats were treated with daily intraperitoneal injection of saline parallel for 8 weeks; ii) DENA-injected group (n=13), in which the rats were intraperitoneally injected with DENA (100 mg/kg body weight; Sigma-Aldrich, St. Louis, MO, USA) twice a week for 8 weeks; iii) taurine plus curcumin treated group (n=10), in which the rats were injected into the stomach with taurine (500 mg/kg body weight) and curcumin (100 mg/kg body weight) daily, with a 1 h interval between the two injections, for 8 weeks; iv) the taurine, curcumin and DENA treated group (n=14), in which the rats were treated with the same dose of taurine and curcumin for one week prior to DENA injection, and received continued treatment [1 ml curcumin (25 mg/100 g body weight) and 2.5 ml taurine (10 mg/100 g body weight] until the day of sacrifice; v) the curcumin-treated group (n=10), in which the rats were injected into the stomach with curcumin (100 mg/kg body weight) daily for 8 weeks; vi) the curcumin plus DENA treated group (n=12), in which the rats were treated with the same dose of curcumin for one week prior to DENA injection, and received continued treatment until the day of sacrifice; vii) the taurine-treated group (n=10), in which the rats were injected into the stomach with taurine (500 mg/kg body weight) for 8 weeks; and viii) the taurine plus DENA treated group (n=12), in which the rats were treated with the same dose of taurine for one week prior to DENA injection, and received continued treatment until the day of sacrifice.
Histopathological analysis
After 8 weeks of treatment, the rats were sacrificed via an overdose of 50 mg/ml ketamine (40 mg/kg; Sigmatech Pharmaceutical Industries, Cairo, Egypt) and sacrificed to obtain liver tissue samples for the detection of HCC. Liver tissue was fixed in 10% normal saline and buffered neutral formalin for 7 days, after which the tissues were washed and dehydrated in ascending grades of ethyl alcohol, cleared using benzene and embedded in paraffin for 1.5 h in the oven at 55°C. Serial sections (5 µm) were cut using a microtome and stained with hematoxylin and eosin, as described previously (40). The tissue sections were then analyzed under a light microscope.
Analysis of liver tumor markers
Serum levels of α-fetoprotein (AFP) were analyzed using an ELISA kit (ShARa/AFP/FITC; Nordic-MUbio, Susteren, The Netherlands). Serum levels of α-L-fucosidase (AFU) were determined using a colorimetric method, according to a previous study (41). Serum levels of the cytokines IL-2 (MBS494288) and IFN-γ (RIF00) were determined using ELISA kits from MyBioSource, Inc. (San Diego, CA, USA) and R&D Systems, Inc. (Minneapolis, MN, USA), respectively.
Electron microscope analysis
The rat livers were removed immediately following sacrifice, and the liver tissue was fixed in 4% formaldehyde and 1% gluteraldehyde in 0.1 M phosphate buffer (pH 7.4) to prepare for electron microscopy analysis, according to a previous study (42). The liver tissue sections were then analyzed under an electron microscope.
Statistical analysis
Data are presented as the mean ± standard deviation. Differences among groups and differences between different time points were analyzed using one-way analysis of variance, followed by Newman-Keuls method, where appropriate. P<0.05 was considered to indicate a statistically significant difference.
Results
Biochemical analysis
The serum levels of AFP and AFU were significantly elevated in the DENA-injected group, as compared with the control group (P<0.001). Conversely, the serum levels of AFP and AFU were significantly decreased in the groups treated with curcumin and/or taurine (P<0.001). However, no significant differences were detected in the serum levels of these markers among the curcumin alone, taurine alone and curcumin plus taurine groups (P>0.05; Table I).
Table I.
Group | Rats (n) | AFP (ng/ml) | AFU (U/l) |
---|---|---|---|
Control | 10 | 1.96±0.67 | 251.9±57.44 |
DENA | 13 | 18.01±1.26 | 669.54±41.08 |
Curcumin | 10 | 0.9±0.43 | 299.46±36.79 |
Curcumin + DENA | 12 | 12.28±0.74 | 312.4±39.93 |
Taurine | 10 | 1.32±0.27 | 295.28±62.67 |
Taurine + DENA | 12 | 7.84±0.69 | 296.55±41.66 |
Taurine + curcumin | 10 | 1.53±0.47 | 175.54±30.66 |
Taurine + curcumin + DENA | 14 | 7.95±0.43 | 271.05±36.99 |
F-value | |||
Control | 0.8 | 1.5 | |
Treatments | 32.94a | 23.18a |
The greater the F-value the greater the significance of difference.
P<0.001. AFP, α-fetoprotein, AFU, α-L-fucosidase; DENA, diethylnitroseamine.
Serum levels of IL-2 were significantly decreased in the DENA-injected group, as compared with the control group (P<0.001). Conversely, the serum levels of IL-2 were significantly elevated in the rats treated with curcumin and/or taurine. Furthermore, the present results demonstrated there were no significant differences in the serum levels of IL-2 among the curcumin alone, taurine alone and curcumin plus taurine groups (Table II and Fig. 1).
Table II.
Group | Rats (n) | IL-2 (pg/ml) | IFN-γ (pg/ml) |
---|---|---|---|
Control | 10 | 251.15±14.39 | 161.59±10.28 |
DENA | 13 | 29.56±2.9 | 16.54±0.91 |
Curcumin | 10 | 229.35±9.87 | 209.17±34.36 |
Curcumin + DENA | 12 | 167.66±13.56 | 109.44±4.74 |
Taurine | 10 | 203.61±9.2 | 181.22±5.83 |
Taurine + DENA | 12 | 173.34±5.63 | 102.73±4.72 |
Taurine + curcumin | 10 | 266.81±12.68 | 171.32±8.01 |
Taurine + curcumin + DENA | 14 | 191.43±4.69 | 122.83±4.89 |
F-value | |||
Control | 1.489 | 1.15 | |
Treatments | 83.44a | 130.92a |
The greater the F-value the greater the significance of difference.
P<0.001. IL-2, interleukin-2; IFN-γ, interferon-γ; DENA, diethylnitroseamine.
Serum levels of IFN-γ were significantly decreased in the DENA-injected group, as compared with the control group (P<0.001), whereas, the serums level of this cytokine were significantly elevated in the curcumin and/or taurine treated groups (P<0.001). In addition, there were no significant differences in the serum levels of IFN-γ among the curcumin alone, taurine alone and curcumin plus taurine groups (P>0.05; Table II and Fig. 2).
Pathological observations
Fig. 3 shows a liver tissue section from a control group rat. It shows a normal liver tissue pattern with normal hepatocytes. A histological observation of liver tissue sections from DENA-injected rats at 4 weeks post-injection showed only mild dysplastic changes, with no obvious malignant changes (not shown). However, at 6 weeks following DENA injection, the tissue sections of the liver showed dysplastic changes, including disorganized hepatic cords (not shown), and liver sections at 8 weeks following DENA injection showed malignant changes associated with HCC grade 1, including nuclear hyperchromasia, irregular anaplasia and pleomorphism, with scattered mitotic figures (Fig. 4).
Liver tissue sections from the rats in the curcumin plus taurine group showed normal liver architecture, with well-preserved hepatocytes (Fig. 5). In addition, the architecture and morphology of the rat liver tissue section from the curcumin plus taurine group were similar to the control group. These results suggest that curcumin and taurine do not have toxic effects on the animal liver.
Electron microscope observations
In the normal control rats, the hepatocytes showed markedly swollen rough endoplasmic reticulum (RER), with few ribosomes attached to their cisternae (Fig. 6). The liver sections of the DENA-treated group showed degenerative cytoplasmic vacuolization, disrupted organelles and malignant changes of the nucleus. In addition, the mitochondria and ER cisternae appeared irregular, and infiltration of inflammatory cells, including macrophages and lymphocytes, were observed in the DENA-treated rat livers (Fig. 7).
In the curcumin-treated group, the number of damaged hepatocytes was unchanged, as compared with the DENA-treated group, although the extent of their internal damage was decreased. Microvilli of the hepatocytes continued to project into the expanded intercellular spaces; however, recovery of the fine structures of the cellular organelles was evident (data not shown). In the DENA-injected group, hepatic necrosis and infiltration of inflammatory cells, particularly into the pericentral region, were observed. However, in the taurine-treated group, these biological changes were diminished and the development of hepatic fibrosis in the pericentral region was inhibited (data not shown).
In the curcumin- plus taurine-treated group, the hepatocytes appeared enlarged and pale, and some exhibited mitotic activity. Furthermore, these cells showed marked dilatation of the RER, indicating some loss of their ribosomes. The periorbital hepatocytes remained unaffected and appeared intact and almost normal. In addition, the nuclei, mitochondria and other organelles were more regular in the curcumin plus taurine group, as compared with the DENA-injected group (Fig. 8).
Discussion
The present study aimed to investigate the protective effects of taurine and curcumin, used alone or in combination, against hepatocarcinogenesis in a DENA-induced rat model of HCC. In the present study, serum levels of AFP and AFU were significantly elevated in the DENA-injected rats, which was consistent with the results of previous studies that demonstrated an association between AFP and AFU serum levels in rats with DENA-induced HCC (43,44).
Conversely, the serum levels of AFP and AFU were significantly decreased in the curcumin-treated rats, thus suggesting a potential protective role of this natural agent against hepatocarcinogenesis. Previous studies have reported anti-inflammatory, antioxidant and anti-cancer activities for curcumin (45–49). The serum levels of AFP and AFU were similarly decreased in the taurine-treated rats, as compared with the DENA-injected rats. These results suggested that taurine may also have a protective effect against hepatocarcinogenesis. Taurine has previously been shown to attenuate or prevent hepatic damage (necrosis and fibrosis) induced by carbon tetrachloride (CCL4) (50–53). Furthermore, in the present study, the serum levels of AFP and AFU were significantly downregulated in the curcumin- plus taurine-treated group, as compared with the DENA-injected group.
The mechanism of action of these two natural compounds is based on the secretion of various cytokines that function via the following pathway: Stimulation of antitumor immune responses, followed by induction of tumor cell apoptosis, inhibition of the uncontrolled proliferation of cancer cells and suppression of angiogenesis (54,55). In the present study, the serum levels of IL-2 and IFN-γ were significantly decreased in the DENA-treated group, as compared with the control group. These results were consistent with a previous study, in which the production of various cytokines, including IL-2, IL-4, IL-5, IFN-γ and TNF-α, was shown to be suppressed in cancer patients (56).
The present study demonstrated that the pathogenic alterations induced by DENA could be inhibited by curcumin treatment. This finding may have been due to the immunomodulatory effect of curcumin, since previous studies have reported that curcumin is a safe and useful immunomodulator (57–60). In the present study, the serum levels of IL-2 and IFN-γ were significantly elevated in the taurine-treated group; thus suggesting that taurine may also exert an immunoregulatory role. This finding is consistent with a previous study, in which taurine was shown to alter the activity of the immune system to protect hepatocytes against membrane disintegration during rat hepatocarcinogenesis (61).
Potential immunoregulatory properties for taurine have been suggested, which are based on the modulation of key proinflammatory cells, in particular neutrophils and T lymphocytes (62). Furthermore, another study using a taurine derivative (taurine chloramines) demonstrated that taurine was able to regulate the synthesis of proinflammatory cytokines, thus suggesting that taurine may have a role in the initiation and propagation of the immune response (63). In addition, a previous study reported that the levels of IL-2 and IFN-γ were markedly elevated following treatment with curcumin and taurine, and were approximately equal to the control untreated levels (64).
In the present study, a histopathological analysis of liver tissue sections from the DENA-treated group at 4 weeks following DENA injection showed mild dysplastic changes and inflammatory cell infiltration. At 6 weeks, the DENA-injected rat liver tissues showed dysplastic changes, including disorganized hepatic tissue. These results were consistent with a previous study, in which hepatocarcinogenesis was shown to be induced in rats at 6 weeks following injection with DENA and weekly subcutaneous injection of CCL4 (65). In the present study, DENA injection for 8 weeks resulted in malignant changes of the liver tissues, including disorganized and irregularly arranged hepatocytes. Furthermore, electron microscopy confirmed the histopathological changes and showed the presence of HCC in the DENA-treated group, as indicated by mitochondrial accumulation, RER stacks and nuclear malignant changes. These results were consistent with a previous study, which demonstrated that normal hepatocytes had fewer mitochondria as compared with HCC cells (66). In addition, HCC cells were shown to contain a greater abundance of, and more widely dispersed, RER.
The present study demonstrated that curcumin effectively inhibited DENA-induced hepatic damage in a rat model of HCC. A histopathological analysis showed moderate improvements in the hepatocellular structure of curcumin-treated rats, as well as the absence of nuclear changes and malignancy. However, some apoptotic changes, such as cell membrane lysis, were observed, which may have been due to the anti-cancer effect of curcumin. These results were in agreement with a previous study that reported direct anti-carcinogenic activities for curcumin (67). Similarly, previous studies demonstrated that curcumin was able to inhibit the proliferation of various cancer cells in culture and prevent carcinogenesis in rodents (29,47,68). In the present study, electron microscopy detected numerous mitochondria, condensed chromatin, small nucleoli and stacks of RER in the hepatocytes of the curcumin-treated rats. These results were consistent with a previous study (69), in which numerous hallmarks of apoptosis, including DNA laddering, chromatin condensation, fragmentation and apoptosis, were observed in curcumin-treated human hepatoblastoma cells.
The present study demonstrated a protective effect for taurine against hepatic damage, as shown by the well-preserved hepatocytes in lung tissue sections from taurine-treated rats. This finding may be due to one or more of the biological roles that have previously been reported for taurine, including antioxidant (free radical scavenging), membrane stabilizing, and immune system potentiation activities (70). Furthermore, these results were supported by a previous study that reported that taurine was a powerful prophylactic agent against hepatocarcinogenesis in rats (71). The electron microscopic observations in the present study suggested that taurine preserves the morphology of major organelles in hepatocytes and delays the development of fibrosis, which was consistent with Tasci et al (72).
In the present study, combined treatment with curcumin and taurine prior to DENA injection resulted in well-preserved hepatocytes and reorganization of venous congestion in the central veins. These findings suggested that the combination of taurine and curcumin exerted a protective effect against hepatocarcinogenesis, which may be due to the anti-cancer activity of curcumin (29), and the anti-neoplastic effect of taurine and its derivatives via inhibition of cell proliferation, induction of tumor cell apoptosis and suppression of angiogenesis (73–77).
In conclusion, the present study demonstrated that combined use of curcumin and taurine exerted a protective effect against hepatocarcinogenesis in a rat model of DENA-induced HCC. Further studies should assess the efficacy of using curcumin and taurine as a potential prophylactic combination therapy against human hepatocarcinogenesis in high-risk groups that are exposed to chemical carcinogens or viral infections, such as hepatitis B or C.
Acknowledgements
The authors would like to thank Mr. Ahmed EL-Husseiny, a research assistant at the Department of Cancer Biology at the National Cancer Institute, Cairo University, for his efforts in supporting the publication of this work, and the staff at the animal care facility for their support in taking care of the animals during the experimental procedures.
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