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. 2017 Jan 17;19(2):135–144. doi: 10.1016/j.neo.2016.12.003

Figure 2.

Figure 2

The interaction between C1QBP and YBX1. (A) Reciprocal co-immunoprecipitation of endogenous C1QBP and YBX1 in SW839 cells. Immunoprecipitates of C1QBP and YBX1 were analyzed by Western blot. (B) Western blot analysis showed that the depletion of C1QBP in SW839 cells increased EGF-induced phosphorylation of YBX1. Cells were deprived of serum for 6 hours before stimulated with EGF at 10 ng/ml for 0, 5, and 15 min. Then the level of YBX1 and p-YBX1 in the cell lysates was analyzed by immunoblotting. (C) Confocal microscopy analysis of C1QBP and YBX1 in SW839 cells. Knockdown of C1QBP enhanced the EGF induced nuclear translocation of YBX1 (yellow arrows).