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NRG1-β activates ERK in MLE-12 cells. MLE-12 cells were incubated with isotonic medium (control, C), or hypertonic medium [+ 200 mOsm sorbitol (S)], NRG1-α (Nα; 10 ng/ml), or NRG1-β (Nβ; 10 ng/ml) for 10 min in the presence of vehicle (1% DMSO), the HER tyrosine kinase inhibitor AG1478 (10 μM), or the PDGF RTK inhibitor AG1296 (20 μM). Cell lysates were probed for P-ERK or total ERK. Sorbitol and NRG1-β produced strong ERK activation; both were inhibited by AG1478.
