Table 1.
ADP-regulated kinases and Tyr-phosphatases
| Early kinases (10 s) | ||||
|---|---|---|---|---|
| Family | Uniprot | Gene (alternative) | Site | Regulation |
| AGC | Q05655 | PRKCD (PKDCδ) | Ser130 | ↑10ADP |
| Thr218 | ↑10ADP | |||
| Ser304 | ↑10ADP | |||
| AGC | Q04759 | PRKCQ (PKCθ) | Thr219 | ↑10ADP |
| Ser685 | ↑10ADP | |||
| AGC | Q13464 | ROCK1 | Ser1341 | ↑10ADP |
| AGC | O75116 | ROCK2 | Ser1374 | ↑10ADP |
| AGC | P23443 | RPS6KB1 (p70S6K) | Ser427 | ↑10ADP |
| CAMK | O60229 | KALRN (TRAD) | Ser1896 | ↑10ADP |
| Ser2261 | ↓10ADP | |||
| Ser1756+1757 | ↑10ADP | |||
| CAMK | P49137 | MAPKAPK2 | Thr334 | ↑10ADP |
| CAMK | Q9BUB5 | MKNK1 (MNK1) | Thr385 | ↑10ADP |
| CAMK | Q7KZI7 | MARK2 | Thr596 | ↑10ADP |
| CAMK | Q15746 | MYLK (smMLCK) | Ser1760 | ↑10ADP |
| Ser1776/Ser1779 | ↑10ADP | |||
| CAMK | Q13555 | CAMK2G | Thr287 | ↑10ADP |
| Thr306 | ↑10ADP | |||
| Ser311 | ↑10ADP | |||
| CK1 | P0C1S8 | WEE2 (WEE1B) | Ser139 | ↑10ADP |
| CMGC | O94921 | CDK14 (PFTAIRE1) | Ser24 | ↑10ADP |
| STE | O94804 | STK10 (LOK) | Ser454 | ↑10ADP |
| STE | Q13043 | STK4 (MST1) | Ser414 | ↑10ADP |
| Ser438 | ↑10ADP | |||
| STE | Q99683 | MAP3K5 (ASK) | Ser1033 | ↑10ADP |
| Ser1240 | ↑10ADP | |||
| Ser1029+Ser1033 | ↑10ADP | |||
| STE | Q13177 | PAK2 | Ser19/Ser20 | ↑10ADP |
| TK | P52333 | JAK3 | Ser15/17/20/Thr21 | ↑10ADP |
| TK | Q06187 | BTK | Thr184 | ↑10ADP |
| TK | Q07912 | TNK2 (ACK) | Thr517/Tyr518 | ↑10ADP |
| TKL | Q8IVT5 | KSR1 | Thr268+Thr272 | ↓10ADP |
| Q8TD19 | NEK9 | Thr333 | ↑10ADP | |
| Q9H4A3 | WNK1 | Ser1261 | ↓10ADP | |
| Medium (30 s) and late kinases (60 s) | ||||
|---|---|---|---|---|
| Family | Uniprot | Gene (alternative) | Site | Regulation |
| AGC | Q05655 | PRKCD (PKCδ) | Thr43 | ↑30ADP |
| CAMK | Q9Y2K2 | SIK3 (QSK) | Ser568 | ↓30ADP |
| CAMK | Q9UEW8 | STK39 (PASK) | Ser385 | ↓30ADP |
| STE | Q13043 | STK4 (MST1) | Thr340 | ↑60ADP |
| STE | Q7L7X3 | TAOK1 (TAO1) | Thr440 | ↑30ADP |
| STE | Q9UKE5 | TNIK (ZC2) | Thr903 | ↑60ADP |
| STE | Q13233 | MAP3K1 (MEKK1) | Thr20 | ↓60ADP |
| STE | Q8N4C8 | MINK1 (MINK/ZC3) | Ser701 | ↓60ADP |
| STE | Q9Y4K4 | MAP4K5 (KHS1) | Thr168 | ↑60ADP |
| STE | O95747 | OXSR1 (OSR1) | Ser359 | ↓60ADP |
| STE | Q13177 | PAK2 | Ser197 | ↑30ADP |
| TK | P43405 | SYK | Ser297 | ↑30ADP |
| TKL | P15056 | BRAF | Ser151 | ↓60ADP |
| Tyrosine phosphatases | |||
|---|---|---|---|
| Uniprot | Gene | Site | Regulation |
| Q05209 | PTPN12 | Thr454 | ↓10ADP |
| Q99952 | PTPN18 | Ser341 | ↑10ADP |
| Q8WYL5 | SSH1 | Ser897 | ↑10ADP |
| Q8TE77 | SSH3 | Ser37 | ↑10ADP |
| Q8TF42 | UBASH3B | Thr106 | ↑10ADP |
| Regulatory subunits of phosphatases | |||
|---|---|---|---|
| Uniprot | Gene | Site | Regulation |
| O14974 | PPP1R12A | Ser478/Ser479 | ↑10ADP |
| Ser903 | ↑60ADP | ||
| O95685 | PPP1R3D | Ser25 | ↑10ADP |
| Q96SB3 | PPP1R9B | Ser99/Ser100 | ↑10ADP |
Kinase phosphorylation sites were grouped into early (10 seconds), medium (30 seconds), and late (60 seconds) responders. ↑10ADP corresponds to upregulation after 10 seconds of ADP stimulation. Involvement of Tyr-phosphatases and regulatory subunits are summarized. If no specific phosphorylation Ser/Thr/Tyr residue could be assigned (eg, JAK3), all possible phospho-residues are listed.