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. 1991 Oct 1;88(19):8357–8361. doi: 10.1073/pnas.88.19.8357

A method for production of antibodies to human T-cell receptor beta-chain variable regions.

Y W Choi 1, B Kotzin 1, J Lafferty 1, J White 1, M Pigeon 1, R Kubo 1, J Kappler 1, P Marrack 1
PMCID: PMC52507  PMID: 1656442

Abstract

Mouse T-cell hybridomas bearing human V beta elements were produced by transfection of human/mouse hybrid T-cell receptor beta-chain genes into a mouse T-cell hybridoma lacking an endogenous beta-chain gene. These hybridomas were entirely mouse in origin except for the human V beta region. These cells were used to immunize mice against human V beta elements. Mouse monoclonal antibodies have thus been generated against human V beta 13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human V beta or V alpha elements.

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Selected References

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