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. 1991 Oct 1;88(19):8377–8381. doi: 10.1073/pnas.88.19.8377

Retroviral-mediated gene transfer into hepatocytes in vivo.

N Ferry 1, O Duplessis 1, D Houssin 1, O Danos 1, J M Heard 1
PMCID: PMC52511  PMID: 1656443

Abstract

Stable gene transfer into hepatocytes might be used to compensate for a genetic deficiency affecting liver function or to deliver diffusible factors into the blood stream. In rats, we have combined retroviral-mediated gene transfer with a surgical procedure in which the liver is temporarily excluded from the circulation and infected in vivo. Partial hepatectomy was performed 24-48 hr before perfusion with virus to induce hepatocyte division and facilitate viral integration. A helper-free recombinant retrovirus coding for beta-galactosidase with nuclear localization was used to score cells that expressed the transgene. For at least 3 months after gene transfer, up to 5% of hepatocytes expressed nuclear beta-galactosidase. Whereas in vitro reimplantation of genetically modified hepatocytes has proved to be inefficient in stably transferring genes into the liver, our approach provides a feasible alternative.

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