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The cytotoxic effects or a lack thereof of secondary amines (P6–P10) on HASMC were determined by LIVE/DEAD assay. The fluorescence was monitored at em 495/ex 530 and em 495/ex 645 using a micro plate reader. Results are expressed as the percentage of live cells and dead cells and mean ± SE. Cells in 1% cDMSO, the vehicle for the amines, served as negative control. * p <0.05 versus 1% cDMSO.
