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. 1992 May 1;89(9):3761–3764. doi: 10.1073/pnas.89.9.3761

Oligonucleotide-mediated triple helix formation using an N3-protonated deoxycytidine analog exhibiting pH-independent binding within the physiological range.

S H Krawczyk 1, J F Milligan 1, S Wadwani 1, C Moulds 1, B C Froehler 1, M D Matteucci 1
PMCID: PMC525570  PMID: 1570294

Abstract

Triple helix formation with pyrimidine deoxyoligonucleotides for the sequence-specific recognition of DNA duplex targets suffers from a decrease in affinity as the pH of the medium increases to that of physiological fluids. A solution to this problem has been identified and entails the substitution of N6-methyl-8-oxo-2'-deoxyadenosine (M) for the 5-methyl-deoxycytosine base residues. The triple helix forming ability of an oligonucleotide consisting of thymidine and M residues is pH independent in the physiological range. Furthermore, M has been found to be superior to the previously used 5-methyldeoxycytidine and deoxyguanosine in conferring increased affinity for duplex DNA under physiological salt conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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