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. 1992 May 1;89(9):3810–3814. doi: 10.1073/pnas.89.9.3810

Fusogenic segments of bovine leukemia virus and simian immunodeficiency virus are interchangeable and mediate fusion by means of oblique insertion in the lipid bilayer of their target cells.

V Vonèche 1, D Portetelle 1, R Kettmann 1, L Willems 1, K Limbach 1, E Paoletti 1, J M Ruysschaert 1, A Burny 1, R Brasseur 1
PMCID: PMC525580  PMID: 1315040

Abstract

Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-terminal segment of gp30 (fusion peptide) into the lipid bilayer in an oblique orientation, as predicted by computer conformational analysis, results in fusogenic capacities higher than insertion in a perpendicular or parallel orientation; and (iii) replacement of the BLV fusion peptide with its simian immunodeficiency virus counterpart does not modify the fusogenic capacity of the BLV glycoprotein.

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Selected References

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